Detection noise significantly degrades the quality of structured illumination microscopy (SIM) images, especially under low-light conditions. Although supervised learning based denoising methods have shown prominent advances in eliminating the noise-induced artifacts, the requirement of a large amount of high-quality training data severely limits their applications. Here we developed a pixel-realignment-based self-supervised denoising framework for SIM (PRS-SIM) that trains an SIM image denoiser with only noisy data and substantially removes the reconstruction artifacts. We demonstrated that PRS-SIM generates artifact-free images with 20-fold less fluorescence than ordinary imaging conditions while achieving comparable super-resolution capability to the ground truth (GT). Moreover, we developed an easy-to-use plugin that enables both training and implementation of PRS-SIM for multimodal SIM platforms including 2D/3D and linear/nonlinear SIM. With PRS-SIM, we achieved long-term super-resolution live-cell imaging of various vulnerable bioprocesses, revealing the clustered distribution of Clathrin-coated pits and detailed interaction dynamics of multiple organelles and the cytoskeleton.

云南具有丰富的矿产资源, 钠长石是当地的优势矿种之一, 然而目前钠长石产业结构单一, 产品附加值低。本文以云南钠长石为原材料, 采用数字光处理(DLP)技术制备钠长石陶瓷, 探究了烧结温度对钠长石陶瓷性能的影响, 并基于研究结果成功制备出结构复杂的钠长石陶瓷。结果表明, DLP技术制备的钠长石陶瓷成形效果良好, 钠长石陶瓷素坯的抗弯强度为18.30 MPa。随着烧结温度的升高, 钠长石陶瓷的主要物相为钠长石和钙长石, 无新相产生, 且钠长石陶瓷的收缩率、致密化程度和抗弯强度逐渐增大。当烧结温度达1 150 ℃时, 钠长石陶瓷试样收缩率最大, 为35.25%(Z方向), 微观组织致密, 力学性能良好, 其抗弯强度为98.69 MPa。将云南钠长石与数字光处理技术相结合制备高性能、结构复杂的钠长石陶瓷对促进云南钠长石的产业发展具有较为重要的推动作用。

High-speed visualization of three-dimensional (3D) processes across a large field of view with cellular resolution is essential for understanding living systems. Light-field microscopy (LFM) has emerged as a powerful tool for fast volumetric imaging. However, one inherent limitation of LFM is that the achievable lateral resolution degrades rapidly with the increase of the distance from the focal plane, which hinders the applications in observing thick samples. Here, we propose Spherical-Aberration-assisted scanning LFM (SAsLFM), a hardware-modification-free method that modulates the phase-space point-spread-functions (PSFs) to extend the effective high-resolution range along the z-axis by ~ 3 times. By transferring the foci to different depths, we take full advantage of the redundant light-field data to preserve finer details over an extended depth range and reduce artifacts near the original focal plane. Experiments on a USAF-resolution chart and zebrafish vasculatures were conducted to verify the effectiveness of the method. We further investigated the capability of SAsLFM in dynamic samples by imaging large-scale calcium transients in the mouse brain, tracking freely-moving jellyfish, and recording the development of Drosophila embryos. In addition, combined with deep-learning approaches, we accelerated the three-dimensional reconstruction of SAsLFM by three orders of magnitude. Our method is compatible with various phase-space imaging techniques without increasing system complexity and can facilitate high-speed large-scale volumetric imaging in thick samples.

空心涡轮叶片是燃气轮机热端的关键零部件，其结构十分复杂，制造难度极大。陶瓷型芯型壳是用于涡轮叶片铸造的重要部件，随着燃气轮机的热端工作温度逐渐升高，所需要的叶片结构也更加精细，传统的精密铸造工艺已经无法满足叶片快速升级换代的需求。激光增材制造技术无需模具，可以加速新产品的研发，缩短制造周期，满足个性化需求。目前可用于型芯型壳制造的激光增材制造技术主要有激光选区烧结和立体光刻两大类。主要介绍了这两大类激光增材制造技术在陶瓷型芯型壳制备方面的应用，从材料配方、素坯成形、后处理烧结等多个方面综述了当前国内外的最新研究进展，探讨了两类技术各自的优势、目前存在的问题以及未来发展的趋势。

This publisher’s note corrects the authors’ affiliations in Photon. Res.8, 940 (2020).PRHEIZ2327-912510.1364/PRJ.389553